Category: xyumcrxlygeq

Traffic Advisory Halifax Regional Municipality

first_img The Sedwidge bridge in Middle Musquodoboit is closed for repairsuntil further notice. Musquodoboit Valley Memorial Hospital can be reached from BarrenRoad or Higginsville Road off Moose River Road. The entrance to Higginsville Road is open to local traffic only. Local Area Office: Middle Musquodoboit 902-384-2599 -30- TRANSPORTATION/PUBLIC WORKS–Traffic Advisory, Halifax RegionalMunicipalitylast_img

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Amendments Introduced to the Social Workers Act

first_img changing the name of the organization from association to college placing appropriate emphasis on the protection of the public as the primary responsibility of the organization updating the definition of social work improving the complaints process to make it more transparent Government introduced amendments to the Social Workers Act on behalf of the Nova Scotia Association of Social Workers today, Nov. 18. This bill will enable the Nova Scotia Association of Social Workers, a self-regulating body, to better regulate the practice of social work in the province. “We are committed to working with social workers to support their profession and provide the tools they need to help protect vulnerable families,” said Community Services Minister Joanne Bernard. Specifically, the amendments include: These changes will bring the profession in Nova Scotia in line with other provinces and reflect current social work objectives and focus. Community Services, as the largest employer of social workers in Nova Scotia, currently employs 450 social workers across the province.last_img read more

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COMMUNITY SERVICESMikmaq Community Projects Receive Innovation Grants

first_img Nearly 100 applications were received from across the province. The names of other communities and organizations receiving grants will be announced soon. Six innovative projects that will help prevent sexual violence in First Nations communities are receiving grants through the province’s Sexual Violence Strategy. Community Services Minister Joanne Bernard today, April 18, announced more than $150,000 in Prevention Innovation Grants for Mi’kmaq organizations during her opening remarks at the Nova Scotia Sexual Violence Strategy Mi’kmaq Community Engagement Gathering in Truro. The two-day gathering is bringing together service providers and community leaders from First Nations communities in Nova Scotia to share knowledge and collectively identify ways communities can prevent and respond to sexualized violence. “The sad truth is that aboriginal women and girls are about three times more vulnerable to sexual victimization than non-aboriginal women,” said Ms. Bernard. “It’s wonderful to see solutions being led by aboriginal community members who know these realities far too well.” The Prevention Innovation Grants are a commitment under government’s Sexual Violence Strategy. There’s an additional $600,000 yet to come for a total investment of more than $1.2 million over two years. Applications for these one-time grants will be made available again in the fall. The grants help community groups and organizations, including youth and underserved populations such as African Nova Scotians, First Nations, and the LGBTQ (lesbian, gay, bisexual, transgender and queer) community, to reach out to their peers and help put creative prevention initiatives into practice. The Nova Scotia Native Women’s Association is receiving more than $36,000 to provide culturally relevant programming to aboriginal women and girls in the Sydney area to enhance skills, bolster confidence and improve the community’s ability to prevent sexual violence. “The project provides our women who have been affected by sexual violence with a connection to their culture,” said Audrey Marshall, of the association. “That gives them a sense of pride and, more importantly, hope.” Other grants were: $46,000 to the Paqtnkek Mi’kmaw Nation in Antigonish County to work with boys in Grades 7 and 8 to explore healthy relationships, consent and positive masculinity $34,000 to the Waycobah First Nation and the Antigonish Women’s Resource Centre to hire a community facilitator to implement a community engagement project on preventing and responding to sexual violence $5,000 to the Mawio’miokuom society of Membertou to facilitate two culturally specific programs for youth, including one that focuses on healthy masculinity and engaging men and boys in ending violence $5,000 to the Millbrook Native Women’s Association for youth to develop a series of public service announcements to help prevent sexual violence $30,000 to the Red Cross to work with First Nations communities in the delivery of culturally sensitive, train-the-trainer sessions on healthy relationships, dating violence, and engaging youth facilitatorslast_img read more

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Toronto Dance Theatre celebrates 50 years

first_img LEAVE A REPLY Cancel replyLog in to leave a comment Twitter Advertisement Login/Register With: Advertisement In the early days, TDT was inspired by the work of iconic American choreographer Martha Graham, in whose company the American-born Randazzo was a principal dancer and with whom both Beatty and Earle studied.But Earle told the Star’s Susan Walker in 1996 that TDT’s technique wasn’t about being slavishly Grahamesque, but “to connect your entire being with your emotional centre. You repeat forms to create connections, then you abandon the forms and keep the connections.”center_img Advertisement Peter Randazzo, foreground, Patricia Beatty and David Earle, founders of Toronto Dance Theatre, in 1988. (DICK LOEK / TORONTO STAR FILE PHOTO) Facebook Back in 1988, the Toronto Star’s William Littler wrote of the then 20-year-old Toronto Dance Theatre that it deserved recognition for having “survived two decades in the volatile world of dance.”Add another three decades to that accolade for what’s been described as Toronto’s first viable modern dance company.The organization, which was founded in 1968 by Patricia Beatty, David Earle and Peter Randazzo, celebrates its 50th anniversary this week under Christopher House, its artistic director since 1994.Christopher House has been artistic director of Toronto Dance Theatre since 1994.last_img read more

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How Google aims to simplify your life with AI

first_imgMOUNTAIN VIEW, Calif. – Google put the spotlight on its artificial intelligence smarts at its annual developers conference Tuesday, announcing new consumer features aimed at simplifying your life.Many of the updates have a practical bent, designed to ease tasks such as composing emails, making lists, navigating city streets and lessening the digital distractions that have increasingly addled people’s lives as a result of previous tech industry innovations.One of the biggest crowd-pleasers for the thousands of software developers who gathered at the outdoor conference was an augmented reality feature on Google Maps that helps people get walking directions. Users will be able to follow arrows — or possibly a cartoon-like creature — that appear on a camera view showing the actual street in front of them.Some new features for Android phones also aim to improve people’s digital well-being, including a new “shush” mode that automatically puts a phone in “do not disturb” mode if you flip it face down on a table. And a “wind down” mode will fade the screen to grey at a designated time to help you disconnect before bed.The company’s digital concierge, known only as the Google Assistant, is getting new voices — including one based on that of musician John Legend — later this year. It will also encourage kids to be polite by thanking them when they say please, similar to a feature Amazon is bringing to its Alexa voice assistant.The assistant may also soon be talking with ordinary people at businesses for tasks such as restaurant reservations, although the feature is still in development.“Hi, I’m calling to book a hair appointment for a client,” said a realistic-sounding automated voice in a demo from the conference stage. The AI assistant deployed pauses and “ums” and “mmm-hmms” to sound more human in conversation with real people.Google said it will roll out the technology, called Duplex, as an experiment in coming weeks. “We really want to work hard to get this right,” said Google CEO Sundar Pichai, who kicked off the conference, known as Google I/O.Other changes are more immediate. Gmail is getting an autocomplete feature that uses machine learning to offer suggestions for finishing half-completed sentences. For example, “I haven’t seen you” might be autocompleted to “I haven’t seen you in a while and I hope you’re doing well.” You can accept the completion by hitting the tab key.The Google Photos app aims to get smarter about suggesting who you might want to share photos with. Whenever it recognizes a photo of one of your Google contacts, it can suggest sharing the photo with that person. It will also convert photos to PDFs and automatically add colour to black-and-white photos or make part of a colour photo black and white. The changes are coming in the next two months.The search giant aims to make its assistant and other services so useful that people can’t live without them — or the search results that drive its advertising business. But it also wants to play up the social benefits of AI and how it’s being used to improve health care, preserve the environment and make scientific discoveries.Pichai didn’t emphasize the privacy and data security concerns that have put companies like Facebook, Twitter and Google in the crosshairs of regulators. But he did say the company “can’t just be wide eyed about the innovations technology creates.”“We know the path ahead needs to be navigated carefully and deliberately,” he said. “Our core mission is to make information more useful, accessible and beneficial to all of society.”Google’s latest version of its Android phone software, for now known as Android P, will offer other smart features. It will teach the battery to adapt to how you use apps in order to conserve energy. Phones using Android P will also learn how to set your screen brightness by studying your manual adjustments, a change from automatic adjustments based on ambient light levels.But not everyone will see the new Android features soon — if at all. Android P won’t be released until later this year, and even then, phone manufacturers and carriers frequently limit Android updates to their newest phones. Owners of Google’s own Pixel phones will get the updates most quickly.Samsung is getting snubbed on some of these new features, at least for now. It has been challenging Google more frequently by launching that services duplicate what Google already offers on Android.For instance, Samsung users won’t have access to an early “beta” version of Android P. Samsung’s camera app also won’t get a built-in Lens feature that lets Google offer information after taking a photo of a building or sign. Samsung has been developing its own similar feature called Bixby Vision.It wasn’t immediately clear which company made the call to withhold the features announced Tuesday.___Anderson reported from New York. AP Technology Writers Matt O’Brien in Providence, Rhode Island and Anick Jesdanun in New York contributed to this report.last_img read more

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Squirrels are destroying some maple syrup operations

DANVILLE, Vt. — Maple syrup producers are contending with a late start to the season in the Northeast and an abundant squirrel population with the critters chomping on plastic sap tubing and spouts.Damage from wildlife — deer, bear woodpeckers, and squirrels — is not unusual for maple operations, but this year squirrels are wreaking havoc in some spots.That means producers must go out into sometimes deep snow to find and replace the damaged lines that transport the sap from the maple trees or other chewed equipment, which producers say can be time-consuming and expensive.Ruth Goodrich of Goodrich’s Maple Farm in Danville, Vermont, the largest maple producing state, says of the squirrels: “Occasionally they declare war. And it seems like they have this year.”The Associated Press read more

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Accomplice of Angoda Lokka arrested in Battaramulla with heroin

Maddumage Chandana Lasantha Perera, alias Angoda Lokka, had been arrested in Dubai recently following the arrest of another notorious underworld leader Makandure Madush. (Colombo Gazette) An accomplice of notorious underworld leader ‘Angoda Lokka’ was arrested in Battaramulla with heroin in his possession.The Police said that the suspect had in his possession 40 grams of heroin at the time of the arrest.

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Katz Group selling Rexall Health with 470 retail pharmacies in 3billion deal

by The Canadian Press Posted Mar 2, 2016 7:39 am MDT AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to RedditRedditShare to 電子郵件Email Katz Group selling Rexall Health, with 470 retail pharmacies, in $3-billion deal REXALL.CA Alberta-based Katz Group has agreed to sell the Rexall Health drugstore chain, including its 470 retail pharmacies, in a C$3-billion deal with U.S. health care company McKesson.McKesson says the friendly deal is the next natural step to take in the relationship between the two companies, which have worked together for 20 years.The San Francisco-based firm previously acquired Katz’s independent outlets and franchise businesses _ primarily operating as I.D.A. and Guardian _ for about C$920 million in 2012.McKesson Canada, which was founded more than 100 years ago, says it would have about 13,000 employees after the Rexall deal closes.The deal is expected to close later this year.The companies say Rexall Health will continue to be led by its current management, headed by CEO Jurgen Schreiber. read more

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MarcAndre Fleury Is Having One Of The Best Playoffs In NHL History

The Flower is already among the eliteGoalies to post at least four shutouts in an NHL playoff run, 1980-2017 And while shutouts are good — they literally guarantee victory in the playoffs — it’s not just in the shutout column that Fleury has impressed: As it stands, he ranks ninth all-time in goals against average for a single playoffs among goalies post-1980 and second all-time in save percentage for a single playoffs among all goalies in the history of the NHL. All this even though the Golden Knights are giving up the most shots per game among teams that are still fighting for the Cup. And despite his reputation as a uniquely bad playoff goalie.That reputation cost Fleury last summer: Pittsburgh didn’t protect him, and the Vegas Golden Knights selected him in the NHL expansion draft. Despite being the No. 1 overall pick in the 2003 NHL Entry Draft — and despite being part of three Penguins teams that won the Stanley Cup — Fleury was effectively cast off to the desert. The Penguins preferred to move forward with their newer, shinier model, Matt Murray. That decision didn’t pay off for Pittsburgh this spring: Murray had the worst playoffs of his young career, recording quality starts in just 50 percent of his games and stopping an abysmal 91 percent of the shots he faced. The top two playoff scorers to this point are Crosby and his teammate Jake Guentzel, yet the Penguins are no longer playing playoff hockey. The problem for Pittsburgh in these playoffs wasn’t putting pucks into the opponents’ net — it was keeping pucks out of their own. If only they’d hung onto that Fleury guy.To be fair to Fleury’s detractors, his playoff numbers before this season are right next to “bad playoff goaltending” in the hockey dictionary: His quality starts percentage in the playoffs is just 50, versus 55 in the regular season. For most of his career, the Flower has experienced a dip in play from regular season to the postseason. This season — for whatever reason — that dip has transformed into a massive ascent. So far this spring, Fleury’s quality starts percentage is an astonishing 80.Fleury’s metamorphosis has been especially impressive when viewed alongside the historically great playoff performances of his goaltending forebears. The majority of goalies on the list above made their mark in a season when scoring was notably down. From 2001-02 to 2003-04, the average goals scored hovered around 2.6 per team per game. It was in this window of dead-puck hockey — right before the NHL lost a season to the lockout — when the top five goalies on this list were tending net. In the 2017-18 regular season, scoring was up to almost 3 goals per team per game during the regular season. The only two goalies on the list who played in a more free-scoring season in this span were Mike Richter and Kirk McLean, who both posted four shutouts in the spring of 1994 en route to the Stanley Cup Final. 2010-11R. Luongo, T. Thomas2.79 GoalieTeamYearShutoutsTeam Result K. McLeanCanucks19944Lost in final M. FleuryGolden Knights20184? N. KhabibulinLightning20045Won Cup M. RichterRangers19944Won Cup Fleury is blanking teams in a high-scoring seasonGoalies to post at least four shutouts in one season’s playoffs and the average goals per game that season, 1979-80 to 2017-18 R. LuongoCanucks20114Lost in final M. BrodeurDevils20037Won Cup 1993-94K. McLean, M. Richter3.24 P. RoyAvalanche20014Won Cup It used to be difficult to imagine hearing “best goalie in the playoffs” and “Marc-Andre Fleury” in the same sentence, unless that sentence read, “Marc-Andre Fleury is most certainly not the best goalie in the playoffs.” But it also used to be difficult to imagine that Alexander Ovechkin would defeat Sidney Crosby in a playoff series, and yet here we are.Up is down, left is right, and Marc-Andre Fleury has been the best goalie in the 2018 Stanley Cup playoffs — and he’s doing it for an expansion team from Las Vegas. And depending on how Fleury plays from now until the end of spring, he might go down as the goalie with the best playoff performance in NHL history.Through his first 10 starts, Fleury — affectionately referred to as “the Flower” by fans and pundits — has pitched four shutouts. For his Vegas Golden Knights to win the Stanley Cup,1We didn’t think they’d win the Stanley Cup, but we didn’t not think they’d win the Stanley Cup either. they have to win eight more games. If Fleury were to continue his current shutout pace of two in every five games — and conservatively assuming that the Golden Knights win each series in a sweep, limiting their netminder’s chances for shutouts — he’d tie Martin Brodeur for the most shutouts in a single Stanley Cup playoffs with seven. If the Golden Knights were to play the maximum number of games they could play for the remainder of the playoffs (14) and if Fleury continued his two in five shutout pace, he would finish with roughly 10 shutouts.And to be clear, shutouts aren’t just the cherries on top of a successful netminder’s playoff sundae — there have been 14 goalie-seasons from 1979-80 to 2016-172Or, since the NHL went to a 16 team playoff format. in which four or more shutouts were posted in a single playoff run, and in six of those, the goalie’s team won the Stanley Cup. 2003-04M. Kiprusoff, N. Khabibulin2.57 Source: Hockey-Reference.com 2001-02D. Hasek, P. Lalime2.62 P. LalimeSenators20024Lost in second round Source: Hockey-Reference.com 1997-98O. Kolzig2.64 E. BelfourStars20004Lost in final SeasonGoaliesAvg. Goals Per Game 2017-18M. Fleury2.97 M. BrodeurDevils20014Lost in final T. ThomasBruins20114Won Cup 2000-01P. Roy, M. Brodeur2.76 O. KolzigCapitals19984Lost in final 2002-03J. Giguere, M. Brodeur2.65 Who knows if the Flower will continue to bloom this spring, but history shows that he’s probably already done enough to earn his Golden Knights a trip to the Stanley Cup Final. And even if Fleury joins Patrick Lalime as the only goalie since 1980 to post four shutouts and not reach the Stanley Cup Final, a conference final berth is a decent consolation prize. And whichever way the pucks bounce going forward, Fleury will have gone further in these playoffs than the team that cast him off last summer. As the Penguins and their younger, shinier goalie march toward the beach, Fleury’s run to the Stanley Cup Final continues. D. HasekRed Wings20026Won Cup M. KiprusoffFlames20045Lost in final 1999-2000E. Belfour2.75 J. GiguereMighty Ducks20035Lost in final read more

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Boy 13 found dead in Doncaster shed fire

first_imgOne user posted along with a photo of them together, saying: “RIP jack my bro. It shouldn’t of been like this. Fly high bro. You didn’t deserve to go like this and will never be forgotten. Love you ladd x (sic).”Another friend: “Rip jack son you meant the world to me and many others, things just aren’t going to be the same without you. You were taken way too soon and you will always be missed I love you son rip (sic).”The same friend also commented that Jack had been filling his bike up with petrol in the shed when something caught on fire. A body of a teenage boy has been found after firefighters were tackling a shed fire in Doncaster, South Yorkshire.Firefighters discovered the 13-year-old’s body after extinguishing the blaze in Campsall, at 9pm last night.The teenager has been named online as Jack Sheldon by friends paying tribute and posting photos of the boy on social media. Want the best of The Telegraph direct to your email and WhatsApp? Sign up to our free twice-daily  Front Page newsletter and new  audio briefings. While another said: “Once again heaven has gained another angle. Rip jack sheldon hearing that he has passed at 13\14 is crazy he had his hole life in front of him! My love and respect go out to his family. Rest in peace bro.”Gone but never forgotten (sic).”Friends also posted “RIP Jack” on his Facebook profile picture and “gone but not forgotten”.An investigation into the death of the teenager has now been launched.A South Yorkshire Police spokesman said: “South Yorkshire Fire and Rescue and South Yorkshire Police attended an address in Byron Avenue, Campsall, Doncaster, at 9pm following a report of a shed fire.”Once the fire was extinguished a body was found within the shed, believed to be that of a 13-year-old male.”Formal identification has not yet taken place and a joint investigation by South Yorkshire Fire and Rescue and South Yorkshire Police is underway to establish the circumstances surrounding this tragic incident.” Fly high bro. You didn’t deserve to go like this and will never be forgottenFriendlast_img read more

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Ménopause bientôt un test pour prédire sa survenue

first_imgMénopause : bientôt un test pour prédire sa survenue ?Iran – Les femmes pourraient bientôt connaître l’âge de leur ménopause, à 4 mois près, grâce un test sanguin mis au point par une équipe de chercheurs iraniens. Le projet a été présenté lundi lors d’une conférence sur la fertilité à Rome et pourrait être un jour utilisé hors laboratoires, si toutefois il s’avère efficace sur un plus large panel.Il permettrait aux femmes désirant fonder une famille de planifier leur(s) éventuelle(s) grossesse(s) grâce à la mesure du taux d’hormone anti-Müllérienne (AMH) dans le sang.Durant l’étude, des chercheurs de l’université des sciences médicales Shahid Beheshti ont fait une prise de sang à un groupe de 266 femmes âgées de 20 à 49 ans tous les 3 ans afin de mesurer leur taux d’hormone AMH.Ce taux baisse en vieillissant, les scientifiques ont donc établi un modèle statistique permettant de prédire l’âge d’arrivée de la ménopause chez une femme. Ils ont prédit grâce à ce modèle statistique l’âge de la ménopause de soixante-trois femmes, en moyenne à 4 mois près, et ont également conclu qu’un faible taux d’AMH à 20 ans conduisait à une ménopause précoce (40-45 ans) alors qu’un taux élevé au même âge se maintenant par la suite aboutit en général à une ménopause après 50 ans. Le 28 juin 2010 à 11:20 • Emmanuel Perrinlast_img read more

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Fuite deau radioactive à la centrale nucléaire de Penly

first_imgFuite d’eau radioactive à la centrale nucléaire de PenlySuite à deux départs de feu dans la centrale nucléaire de Penly, EDF annonce avoir constaté “une fuite d’eau radioactive”.  Inquiétude en Seine-Maritime. A quelques kilomètres de Dieppe, la centrale nucléaire de Penly est au centre de toutes les attentions depuis le jeudi 5 avril 2012, 12h20. C’est à cette heure précise que deux départs de feu ont été constatés. Les pompiers rapidement sur place sont parvenus à maitriser les flammes. Il n’y eu aucun blessé, mais EDF a été contraint de suspendre l’activité d’un réacteur. Pire, dans la soirée, l’entreprise a indiqué via un communiqué de presse avoir constaté une fuite d’eau radioactive. “un défaut a été identifié sur un joint de l’une des quatre pompes de refroidissement du circuit primaire dans le bâtiment réacteur de l’unité de production numéro 2, provoquant une fuite d’eau collectée dans des réservoirs prévus à cet effet”. Selon ce même communiqué la fuite est collectée dans des réservoirs prévus en cas d’incident et le réacteur continue de manière normale son cycle de refroidissement.À lire aussiLe pied d’éléphant, cette effrayante masse radioactive cachée dans les entrailles de TchernobylSelon EDF, cette fuite “n’a aucune conséquence sur l’environnement”. Pour autant, l’Autorité de sûreté nucléaire (ASN) annonce qu’elle “mobilise ses équipes dans son centre d’urgence parisien”. Contacté par l’AFP,Le directeur général de l’ASN Jean-Christophe Niel a précisé que “le volume de la fuite qui était de 2,3 mètres cube par heure en début de soirée avait baissé à 0,1 mètre cube (une centaine de litres) par heure. De l’eau est injectée dans le circuit primaire pour compenser la fuite, et le circuit primaire continue de la sorte à assurer son rôle dans le refroidissement du réacteur”.Depuis midi, la lumière se fait peu à peu sur les causes de l’incident. Le feu aurait pris sur deux petites flaques d’huile. Invité sur France 3, en début de soirée, Laurent Lacroix, directeur délégué de la centrale nucléaire de Penly expliquait: “Nous faisons en ce moment même un diagnostic de l’état des installations pour établir les causes de cet événement et pouvoir redémarrer l’installation. Nous sommes en train d’établir les causes de la présence de ces flaques d’huile à cet endroit. Les investigations en cours permettront de déterminer pourquoi elles étaient là et comment elles se sont enflammées”.  L’évènement rappelle celui survenu à la centrale nucléaire de Marcoule en septembre 2011. Cet incident intervient à quelques jours des élections présidentielles. Alors que les évènements de Fukushima avaient replacé le sujet du nucléaire au centre des débats, la campagne avait quelque peu éclipsé la question. Nul doute que cette fuite d’eau radioactive suscitera de nombreuses réactions politiques. Le 5 avril 2012 à 23:53 • Maxime Lambertlast_img read more

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Tuchel PSG job not dependent on success in Europe

first_imgParis St Germain manager Thomas Tuchel has revealed that his job is not dependent on Champions League success.The Parisians have invested heavily in the squad in a bid to end the Champions League hoodoo, but the current French Champions have never progressed beyond the quarter-finals.“No-one has told me this, in all the talks, I have to win this otherwise I didn’t do my job right,” Tuchel said.“If anyone had told me (that) I wouldn’t have signed the contract.Roberto Firmino, LiverpoolVirgil van Dijk praises Roberto Firmino after Liverpool’s win Andrew Smyth – September 14, 2019 Virgil van Dijk hailed team-mate Roberto Firmino after coming off the bench to inspire Liverpool to a 3-1 comeback win against Newcastle United.“If we judge ourselves only by the biggest goal in European football, the possibility of a big disappointment is high. Why should we do this?“Sometimes I feel sorry for my team because I know the effort they put in. We play at home against defensive teams and we win and win and win.“It is not because it is easy, it is because these guys show the hunger to win and win and win. It is a point of view (that Ligue 1 is easy) that does not help us within the club.”last_img read more

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Peter Tork Of The Monkees Dies At 77

first_imgTork was known for his musicianship and comedic presence in the ’60s television sensation groupNate HertweckGRAMMYs Feb 21, 2019 – 3:51 pm Peter Tork, multi-insturmentalist and actor most famous for his work ’60s television musical sensation the Monkees has died. He was 77. Facebook “There are no words right now… heartbroken over the loss of my Monkee brother, Peter Tork,” bandmate Mickey Dolenz tweeted.Prior to the explosion of popularity the Monkees experienced during their brief but impactful initial run from 1966 to 1971, Tork was part of New York’s Greenwich Village folk scene in the early ’60s.“To be in Greenwich Village in the ’60s was pure joy; to be young was pure bliss,” Tork once told UK Music Reviews. “When I talk about having a well favored life, I went from Greenwich Village almost directly into The Monkees. … I had first heard about the Monkees in the early summer of 1965 from a good friend of mine, one Mr. Stephen Stills.”After Stills introduced him to his future group, they went on to become a national craze on their way to earning an Emmy Award for Outstanding Comedy and four GRAMMY nominations, two each for their enduring hits”Last Train To Clarksville” and “I’m A Believer.” Tork was a man of many talents, playing several instruments in the band as well as contributing vocals and his unique and endearing brand of comedy.”Peter Tork died this a.m.,” wrote bandmate Michael Nesmith. “I am told he slipped away peacefully. Yet, as I write this my tears are awash, and my heart is broken. Even though I am clinging to the idea that we all continue, the pain that attends these passings has no cure. It’s going to be a rough day. I share with all Monkees fans this change, this ‘loss,’ even so. PT will be a part of me forever.”Dolenz, Nesmith, and Tork reunited for a U.S. tour in 2012, following their fellow Monkee Davy Jones’ death. The shows marked their first live performances together in 15 years.While no cause of death has been revealed, Tork was diagnosed with a rare form of cancer a decade ago. His legacy will live on in the music, fans and friends he made during his life.Billie Holliday Documentary In The WorksRead more Twitter Peter Tork Of The Monkees Dies At 77 center_img Peter Tork Of The Monkees Dies At 77 peter-tork-monkees-dies-77 News Email last_img read more

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Unusual Seismic Activity Continues Near Noatak

first_imgMap for April and May 2014 Earthquakes in Northwestern Alaska. (Image courtesy Alaska Earthquake Center)Strong earthquake activity continues near Noatak, with a strong aftershock recorded last week that has become the third powerful temblor in what is now a series of strong quakes and potent aftershocks in just the last two months.Download AudioNatasha Ruppert, a seismologist with the Alaska Earthquake Center in Fairbanks, said Thursday’s 4.6 magnitude aftershock struck just before 10:30 p.m. about 18 miles northeast of Noatak.“This one [is] in almost exactly the same area, so this one was another large aftershock,” Ruppert said—a lingering jolt in the wake of the powerful 5.6 magnitude rumble felt April 18, the strongest quake in the region in more than 30 years.“They all are in the same area, but basically the main shock was … in April 18,” Ruppert said. “There was another large magnitude 5.5 earthquake [on May 3.]” The April quake was followed by nearly 100 aftershocks (represented by blue circles in the above image), five of which were magnitude 4.0 or stronger and one that registered a 5.3 magnitude. The early May quake, though slightly weaker than its April predecessor, was similarly followed by strong aftershocks, seven of which were of magnitude 4.0 or more.After that earlier May quake, the Earthquake Information Center traveled to Kotzebue and Noatak to install sensors closer to the activity.“We are able to tell, now with new equipment, we are actually seeing that these events are a little bit more shallower, closer to the surface, than what we thought before,” Ruppert said. “We are able actually to compute locations for these earthquakes much more accurately,” as a result of the new sensors, she added.Ruppert said the remote nature of the far western Brooks Range means there’s still no firm scientific consensus for what faults are causing earthquakes and other tectonic activity in that region of western Alaska.More accurate readings on these quakes and aftershocks, she said, could help build that consensus.“This particular earthquake sequence is just another piece of the tectonic puzzle that we are trying to build for that region,” she said.As part of their summer fieldwork, Ruppert said a team from the Earthquake Center will be visiting Nome this July for continued work in western Alaska.last_img read more

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Spanish football club chief who believes in flat Earth theory challenges experts

first_imgRepresentation of flat earthScreengrab from YouTubeJavi Poves, the president of a Spanish soccer club, recently changed the name of his team from Móstoles Balompié to Flat Earth FC. Through this move, Poves was aiming to promote the ‘Flat Earth’ conspiracy theory which recently gained widespread popularity among several celebrities and sports personalities.In a recent talk with the Spanish radio station Cadena Ser on Monday, Poves argued that there is a huge economic motive behind the globe Earth theory. He also offered a reward to scientists who succeed in submitting a photograph of the space that is not made with the help of computer graphics.”There is a huge economic motive for which many people want to convince us that the Earth is round. If the Earth is spherical, why are so many people scared? There is a cool reward for whoever gets a photo of space that isn’t CGI. I have never felt the Earth move. I’m still waiting for you to show me: how does water curve?” said Poves during the talk, Newsweek reports.Poves also argued that Spanish science minister and former astronaut Pedro Duque will not take up this challenge as he is afraid of losing the contest. The football club president also alleged that Pedro Duque is earning a lot of money by spreading white lies about the shape of the Earth.As the name suggests, believers of flat Earth theory believe that the planet in which we live in is disc-shaped. These conspiracy theorists argue that snow walls in Antarctica act as a wall to protect living beings from falling.Javi Poves is not the first sports personality to propagate the flat Earth theory. A few months back, Andrew Flintoff, former English cricketer had claimed that the Earth is flat and not spherical. To substantiate his theory, Flintoff also cited an outlandish example.”If you’re in a helicopter and you hover why does the Earth not come to you if it’s round? Why, if we’re hurtling through space, why would water stay still? Why is it not wobbling? Also if you fire a laser about 16 miles, if the world was curved, you shouldn’t be able to see it but you can,” said Flintoff.last_img read more

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4 more killed in alleged gunfights in 3 dists

first_imgProthom Alo IllustrationFour people were killed in alleged gunfights with law enforcement agencies in Khulna, Rajshahi and Jashore districts early Wednesday.Among the deceased, two were killed in Jashore in what police called shoot-out between suspected rival robber groups while the other two were killed in reported gun battles with RAB in Khulna and Rajshahi, reports UNB.Two of the deceased are drug suspects Emran alias Rocky, 22, son of a top drug trader Sheikh Mizanur Rahman alias Hatkata Mizan from Maheshwarpur in Daulatpur upazila of Khulna and Sazzad Hossain, son of one Saim Uddin of Haripur Banpara village in Poba of Rajshahi.With the latest deaths, at least 148 people were killed in reported gunfights with members of law enforcement agencies while 37 bodies of suspected drug traders were recovered after alleged gun battles between rival groups during the countrywide anti-narcotic drives since 15 May.In Jashore, police recovered bullet-hit bodies of two suspected robbers on Jashore-Manirampur road in Kanaitala of sadar upazila around 2:55am.Acting on secret information that two gangs of robbers were exchanging gunfire, a team of police went in the area, recovered the bodies and sent those to Jashore General Hospital, Kotwali police station officer-in-charge Apurba Hasan said.The law enforcement also recovered three sharp weapons and rope from the spot, he added.In Khulna, Emran alias Rocky was killed in a reported gunfight with members of Rapid Action Battalion (RAB) in Maheshwarpur of Daulatpur upazila.Tipped off, a team of RAB-6 gave a signal to three people riding a motorcycle to stop in the area around 3:00am, said ASP Bazlur Rashid of RAB-6.The motorcyclists did not stop, rather opened fire aiming the RAB men, forcing them to fire back in self-defence, he said.After a brief gun battle, RAB men recovered the body of Emran along with a revolver, three bullets and 308 pieces of yaba tablets from the spot, Bazlur Rashid said.Emran was wanted in seven cases, the RAB official said.In Rajshahi, Sazzad Hossain was killed in an alleged gunfight with RAB in Kasba of Poba upazila early Wednesday.Tipped off, a team of RAB-5 conducted a drive in the area around 1:00am. Sensing the presence of the force, the drug suspects opened fire on them, which prompted the RAB members to swing into action, RAB-5 deputy commanding officer major AM Ashraful Islam said.After a brief gunfight, RAB members recovered the body of Sazzad and sent it to Rajshahi Medical College Hospital morgue, he added.Sazzad was wanted in 7/8 cases, major AM Ashraful Islam said.Prothom Alo, however, could not verify the law enforcement’s versions of the incidents independently as no versions of the incidents were available immediately either from any witnesses or from any member of the victims’ families.last_img read more

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5000YearOld Chinese Beer Had This Special Ingredient

first_imgStay on target Fiber-Based Six-Pack Can Rings Offer Eco-Friendly Alternative to PlasticEven More Kellogg’s Cereal Gets Re-Born as Craft Beer If you’re a big beer drinker, you might be interested in knowing what made to beers of yesteryear tick, or at the very least what went into their creation. As it turns out, a Chinese recipe for beer that’s 5,000 years old has been pieced together from prehistoric pots, and they include a special ingredient: barley.After scientists ran several tests on the ancient pottery and other items found at the Mijiaya archaeological site in the Chinese province of Shaanxi. They came back with traces of oxalate. This is a byproduct that creates a special scale known colloquially as “beerstone” that forms on the equipment used to brew beer. There were also different residues from plants like barley.It’s interesting news because researchers weren’t exactly clear on when beer started being made in China, and the earliest known usage of using barley is predated by about 1,000 years. The assumption is, following those ideas, that barley could have been utilized to make beer long before China took the grain and used it as an important part of the citizens’ diets. You’ve gotta consider that barley is still widely used today as well.Of course, barley was already in wide rotation across the rest of the world, such as in ancient Egypt, and it’s possible the widespread use could have trickled into China since it was such an important part of brewing beer and a new crop at that.Scientists have found success when it comes to reconstructing the old recipe, which is ridiculously impressive given how far back it was created. The process and the idea that things could have worked out like this is massively interesting. And it seems like the ancient Chinese civilizations really dug their beer. You probably have something in common with them.last_img read more

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Gotham Wraps Up the First Half of Mad City a Little Too

first_img NYCC 18: The Cast of Gotham On Bane, Batman and Saying GoodbyeDC TV Comes to NYCC, Joaquin Phoenix’s Joker Grin & More DC News Stay on target Can you believe Gotham has already reached it’s halfway point for the year? This year’s midseason finale has its flaws but still turned out to be one of the better episodes this season. Now that we know that Mario is infected with Alice Tetch’s blood, it’s no surprise that he’s hassling the doctor who came up with a test for the virus. Well, he does more than that. He imagines the doctor is Jim Gordon and crushes his head with his bare hands. The next morning, Jim and Bullock investigate the crime scene. Gordon thinks Barnes had something to do with the murder and Bullock says the stress of Lee’s wedding that day is causing Gordon to see things that aren’t there. Until Gordon figures out that the doctor was testing Alice’s blood, that is.Gordon investigates the doctor’s lab and finds that his keycard was just used 20 minutes ago. He instructs the security guard to call the police and runs to the lab. He finds a dead doctor on the ground before being attacked by Mario. After knocking Gordon out, Mario says he plans to make Lee hate Gordon before he kills him. He writes the word, “Arkham” on Gordon’s hand. After coming to, Gordon goes to Arkham Asylum to speak with Jervis Tetch, the Mad Hatter. He demands the name of the person Tetch infected with Alice’s blood. He traps Tetch into giving up a clue by repeating rhyming riddles and figures out that Mario has been infected.He has Mario brought into GCPD for testing, but the test comes back negative. Gordon reasons that Mario broke into the lab to figure out how to beat the test. Gordon goes to Lee’s house to warn her and finds Mario waiting for him. Mario says that he’s afraid that a part of Lee still loves Gordon. When Gordon tries to make Mario come with him, Mario reminds him that he’s Carmine Falcone’s son. Victor Zsasz appears behind Gordon, holding him at gunpoint. It turns out to only be a temporary setback as Zsasz was only instructed to hold him for a while.Morena Baccarin as Lee (Credit: Jeff Neumann/FOX.)Gordon runs to Lee and tries to warn her about her fiance. She knows he thinks Mario has Alice Tetch’s virus and she think’s he’s just acting out of jealousy. Gordon realizes that that’s exactly what Mario wanted and Gordon confesses to Lee that he loves her. He tells her everything that happened after he got out of prison and asked her not to marry him. Lee hates him for telling her all that and says that after they get married, they’re leaving Gotham and never coming back. As Lee marries Mario, Gordon tries to off Carmine Falcone’s goons, but they outnumber and overpower him.Lucius figures out how a person could beat the test and finds that Mario’s blood contains high levels of a drug that can mask the virus. Bullock gets a search warrant and calls Gordon. Once they find proof that Mario covered up the virus, Gordon tries to contact Lee and finds out that she and Mario ducked out of the reception early. He convinces Carmine Falcone to tell him where they went, promising to bring him in alive.At a secluded Cabin, Mario asks Lee to assure him that she doesn’t still love Gordon. Lee says that a part of her will always care for him, she loves and married Mario. Mario pretends to be OK with that, but the rage starts boiling up inside. Gordon arrives just as Mario is about to stab Lee with a knife. He shoots Mario in the chest, breaking his promise to Falcone. The knife falls off the deck into the water and is washed away.Camren Bicondova as Selina Kyle and David Mazouz as Bruce Wayne (Credit: Jeff Neumann/FOX.)Meanwhile, Bruce and Alfred learn that Volk and Dimitri have been killed by Talon, the Court of Owl’s assassin. Alfred reasons that the Court already knows Bruce has gone back on their deal. Bruce vows to do what he can to take the court down. They plan to break into the Court-controlled building that contains the safe later that night. Selina is obviously worried about Bruce, but she says it’s just hunger. Later, she tells Alfred that she felt like they were being watched.Alfred relates Selina’s fears to Bruce and Bruce thinks she’s just mad that he told Ivy they were dating. Alfred is about as tired of the teenage romantic drama as we all are. That evening, when the Whisper Gang doesn’t show up, Alfred is about to go home. Selina shows up and offers to break into the building. Once inside, she has Bruce hang on to a tightrope so she can bypass the alarmed floor and open the safe. Inside, she finds a glass owl. Just as she grabs it, Talon attacks Bruce. Bruce uses an explosive charge, allowing him and Selina to escape. Alfred meets back up with them and tries to fight off Talon. Just as Talon gets the upper hand, a mysterious woman hits him from behind with a vase. She reveals her face and Selina recognizes the woman. She’s her mom.Nygma still hasn’t figured out that Penguin is responsible for Isabella’s death. He thinks the culprit is one of the mayor’s enemies seeking to weaken Penguin by weakening his right-hand man. The Riddler sure sucks at riddles when he doesn’t want to see the answer. Barbara gives him a helpful push via an obviously fake “Penguin!” cough. Nygma doesn’t believe that Penguin killed Isabella, or that he’s in love with him. Barbara asks if he doesn’t owe it to Isabella to find out.Cory Michael Smith as Edward Nygma. (Credit: Jeff Neumann/FOX.)Nygma tests the theory by resigning from his post and explaining to the Penguin that he wants to become more than friends. The Penguin is overjoyed by the news and confesses his love for Nygma. Nygma stops him, explaining that he was only talking about becoming business partners and leaves. Later, after fantasizing about stabbing Penguin in the neck, he tells Penguin to remember that he was his best friend. He then meets up with Barbara, Tabitha, and Butch to make a deal. They help him destroy Penguin, and he helps Barbara become the queen of Gotham’s underworld.While this wasn’t the most exciting midseason finale that Gotham has had, it was a great episode nonetheless. It had some great tension, brutal fight scenes and set some high expectations for the second half of the season. The war between Riddler and Penguin is now in full swing; Gordon made himself an enemy of Falcone (and probably Lee) and Bruce is about to take on the Court of Owls head on. The final scenes felt rushed, though. Maybe if the first few episodes of the season hadn’t been so slow, they would have had more time to reach the cliffhanger more organically. As it is now, it felt too easy for Gordon to find where Lee and Mario had gone, and a little implausible that he’d get there at that exact moment. But this is Jim Gordon we’re talking about. He’s never had the best luck or timing.John Doman as Carmine Falcone. (Credit: Jeff Neumann/FOX.)Gotham‘s last couple midseason finales felt like the stakes were much higher. They felt like events. This one didn’t quite accomplish that, but still managed a very good episode in its own right. And hey, it did its job as far as setting up the back half of the season. I can’t wait to see how crazy things get when the show returns in 2017.last_img read more

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Isolating intact bacteria from blood using a microfluidic monolith device

first_img DLS measurement of (a) initial 25× diluted blood, chemically lysed blood, and blood lysed by perfusion through the monolith device, revealing a significant reduction in cell debris size for mechanical monolith lysis over chemical lysis. (b) DLS measurement of E. cloacae suspended in 1× PBS, and sample perfused through the monolith device, showing no change in bacteria size. (c) DLS measurement of 100× diluted blood spiked with E. cloacae, and sample collected from the outlet of a porous monolith. The broad peak in the inlet sample indicates a mixed population of blood cells and small bacterial cells, whereas the outlet sample showed significant reduction in large (>2 µm) cells, as confirmed in the optical images. Scale bars = 25 µm. Credit: Microsystems & Nanoengineering, doi: 10.1038/s41378-019-0063-4 More information: Jung Y. Han et al. Isolation of intact bacteria from blood by selective cell lysis in a microfluidic porous silica monolith, Microsystems & Nanoengineering (2019). DOI: 10.1038/s41378-019-0063-4 Susanne Pahlow et al. Isolation and identification of bacteria by means of Raman spectroscopy, Advanced Drug Delivery Reviews (2015). DOI: 10.1016/j.addr.2015.04.006 Joo H Kang et al. An extracorporeal blood-cleansing device for sepsis therapy, Nature Medicine (2014). DOI: 10.1038/nm.3640 Anna K. Boardman et al. Rapid Microbial Sample Preparation from Blood Using a Novel Concentration Device, PLOS ONE (2015). DOI: 10.1371/journal.pone.0116837 Han et al. modified previously reported silica monolith synthesis processes, followed by hydrolysis and condensation of silica to form silica glass at low temperatures. To prepare the silica monolith, the scientists used a precursor solution composed of alkyl silicates, polyethylene glycol (PEG) as a porogen, urea as a source of hydroxyl ions to minimize heterogeneity and acetic acid. When they optimized the synthetic process, the resulting monoliths were homogenous and well-anchored to the silica capillary walls. The scientists measured the thickness of the final skeletal monolith structure and calculated its permeability using high-performance liquid chromatography to control experimental conditions. To minimize intrinsic variation, Han et al. cut the resulting capillary tubes into 5 cm long segments to test permeability before use. This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. How bacteria kill host cells from the inside (a) Integration of a silica monolith brick into a thermoplastic chip. A circular tape is placed on a monolith inserted into a COP substrate, and solvated COP is applied to the exposed surface. After partial drying, the tape is removed, the device is enclosed by another COP substrate, and fluid ports are inserted into holes that provide a flow path through the monolith. (b) SEM image of a monolith brick cut by wafer dicing saw. (c) Image of a device during whole blood perfusion. Credit: Microsystems & Nanoengineering, doi: 10.1038/s41378-019-0063-4 (a) RBC lysis efficiency of whole blood in high-throughput devices following perfusion at 10 μL/min (EC E. cloacae, LL L. lactis, BS B. subtilis. Error bars are ±SD. N = 3 for blood and B + EC, and N = 2 for B + LL, B + BS. (b) Blood cell lysis and bacterial separation following serial operation using two monoliths. Surfaces were passivated with BSA/Tween 20. Over 99.999% RBC lysis was obtained while preserving viability of L. lactis and E. cloacae. Scale bars = 100 µm. c Raman spectra of whole blood spiked with E. cloacae (upper) before and (under) after processing through porous silica monolith. Credit: Microsystems & Nanoengineering, doi: 10.1038/s41378-019-0063-4. They then developed two complementary methods for low and high-throughput operation to integrate silica monoliths into microfluidic systems. To allow low throughput operation, the scientists embedded monolith-containing capillary segments within thermoplastic microfluidic chips to protect the monolith during integration. For high throughput-selective lysis they used monoliths with larger cross-sectional areas within the microfluidic devices. The complete method of fabrication yielded excellent reliability for leak-free operation during whole blood perfusion. , PLoS ONE They explored the impact of monolithic morphology, geometry and flow conditions on cell lysis and flow regimes that allowed selective cell lysis and selective passage of multiple gram negative and gram positive bacteria. The technique employed by Han et al. allowed rapid sample preparation and bacterial analysis when combined with single-cell Raman spectrometry. The work provides unique sample preparation steps to support rapid and culture-free bacterial analysis for applications in point-of-care biomedical devices. Bacteria in blood can lead to sepsis, infection of tissues and other serious medical conditions, requiring early identification of blood-borne bacteria for effective treatment. The ability to identify bacteria rapidly using point-of-care diagnostics can greatly enhance clinical potential for optimal treatment during early-stage infection. The existing gold standard for bacterial characterization is based on phenotypic cell culture analysis and requires at least 24 hours to collect samples for culture and analysis in a diagnostic and clinical microbiology lab. The existing technique is robust and inexpensive but cannot generate timely results to guide the initial stages of treatment. As proof-of-principle, Han et al. selected Enterobacter cloacae (gram-negative, rod shaped bacteria) to explore their efficacy of passage, alongside three gram-positive bacteria; Lactococcus lactis, Micrococcus luteus and Bacillus subtilis. During the experiments, they perfused bacterial solutions through the microfluidic monoliths with varying geometry and flow conditions to test the passage of bacteria and blood cell lysis using dynamic light scattering (DLS). For instance, the perfusion of purified E. cloacae through the monolith did not yield discernable changes in the DLS peaks, indicating the intact passage of bacteria. The scientists showed the effect of the length of the porous monolith device on the efficiency of red blood cell lysis (RBC). The results indicated that RBC lysis efficiency increased significantly for monolith lengths above 1 mm. Han et al. also studied the fate of white blood cells (WBCs) during operation of the monolith device, the cells could not pass through the monolith without being lysed similar to RBCs. Technically, RBCs deformed to a discoid shape to pass through the monolith, which caused significantly increased membrane tension to result in RBC lysis. Comparatively, bacterial cells had similar dimensions to the monolith pores and therefore required less cell wall expansion for successful passage without rupture. The scientists optimized the parameters of the device for diverse bacteria to tolerate high levels of membrane stress without rupture. Further developments ensured the intact passage of bacteria without degradation and with viability.center_img For high-throughput bacterial passage, the scientists diluted the blood in the capillary devices. However, as an alternative, they could also extend the capacity of monoliths for whole blood lysis. The devices processed more than 400 µL of whole blood spiked with bacteria before exhibiting a significant increase in back pressure, due to clogging as a result of cell lysis and also due to intact leukocytes (WBCs) trapped within the porous matrix.To locate target bacteria, Han et al. obtained a sample deposited on to a glass slide, after it passed through the monolith-process. They conducted single-cell Raman analysis by manually scanning the optical probe across the sample. They expect the use of selective lysis technology, coupled to confocal Raman microscopy in the future to improve the process of detecting bacterial strains of interest at low concentrations in a defined location of interest. In this way, Jung Y. Han and colleagues developed a microfluidic monolith to efficiently isolate intact bacteria with wide-ranging theranostic, point-of-care potential for clinical applications. They envision the union of confocal Raman microscopy tools that are currently largely confined to the research lab with emerging miniaturized and handheld systems to pave the way towards rapid and portable point-of-care devices. TOP: (a) Synthesis of porous silica monolith in fused silica capillary and thermoplastic mold via sol–gel chemistry. (b) Competitive reactions during the synthesis of monolith. Silanol groups present on glass capillary form covalent bonds with the monolith during this step. BOTTOM: (a) SEM image of a silica monolith synthesized within a 100 μm ID fused silica capillary, revealing uniform porosity and excellent wall anchoring of the monolith. (b) Histogram of pore size. Critical diameter for RBC hemolysis (2r*) is marked with an arrow. Credit: Microsystems & Nanoengineering, doi: 10.1038/s41378-019-0063-4 Emerging single-cell diagnostics rely on the potential to rapidly and efficiently isolate bacteria from complex biological matrices. In a recent study now published in Microsystems and Nanoengineering, Jung Y. Han and colleagues at the interdisciplinary Departments of Mechanical Engineering, Chemical Biomolecular Engineering and Bioengineering in the U.S. developed a device to isolate intact and viable bacteria from whole blood using a microfluidic, porous silica monolith. They achieved mechanical hemolysis while providing passage of intact and viable bacteria through the monoliths for size-based bacterial isolation and selective lysis. Han et al. described a process to synthesize large quantities of discrete capillary-bound monolith elements and millimeter-scale monolith bricks to integrate into microfluidic chips. In the present work, Han et al. explored microfluidic devices integrated with porous silica monoliths as simple flow-through elements for selective blood cell analysis and the intact isolation of bacteria. Monoliths are highly porous materials composed of open cell morphology with twisting paths of fluid flow. Scientists can control monolithic pore morphology via high mechanical surface stress during cell perfusion for mechanical hemolysis of blood cells, while allowing intact and viable bacteria to travel the winding flow paths for their culture-free isolation. Han et al. used the approach of selective passage for bacteria in whole blood under flow conditions for gram positive and gram-negative species, despite differences of the bacterial strains. The technique of high-throughput selective monolith lysis combined with powerful analytical methods such as Raman spectroscopy can allow culture-free analysis of bacteria in whole blood at the level of the single cell. (a) Monolith length dependence of RBC hemolysis. Totally, 50× diluted blood in 1× PBS was perfused through capillary monoliths of various lengths at a flow rate of 10 μL/min. (b) Passage rate of RBC and viable bacteria at different flow rates and lengths of monolith-containing capillary. Scale bars = 50 µm. Error bars are ±1SD. Contrast of optical images was adjusted for visibility. Credit: Microsystems & Nanoengineering, doi: 10.1038/s41378-019-0063-4 Journal information: Nature Medicine © 2019 Science X Network Citation: Isolating intact bacteria from blood using a microfluidic monolith device (2019, July 2) retrieved 18 August 2019 from https://phys.org/news/2019-07-isolating-intact-bacteria-blood-microfluidic.html Explore furtherlast_img read more

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